DNA

Part:BBa_J100121:Design

Designed by: Phoebe Parrish, Meredith Simpson, A. Malcolm Campbell   Group: Campbell M Lab   (2013-07-31)


Promoter + Spinach + BsaI sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 553
    Illegal PstI site found at 256
    Illegal NotI site found at 205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal PstI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 246
    Illegal BsaI site found at 266
    Illegal BsaI.rc site found at 20


Design Notes

This part was designed using iPCR to amplify the plasmid sent to us by CMU, removing FAP and the RBS. The iPCR primers contained BsaI and PstI sites with spacers in between. The iPCR products were digested using PstI, then ligated together.


Source

The sequences were given to us by the Carnegie Mellon University 2012 iGEM team (http://2012.igem.org/Team:Carnegie_Mellon).

References